LOCUS pRS413 4970 bp DNA circular 22-OCT-2007 DEFINITION Cloning vector pRS413, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pRS413 ORGANISM Cloning vector pRS413 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4970) AUTHORS Sikorski,R.S. and Hieter,P. TITLE A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae JOURNAL Genetics 122 (1), 19-27 (1989) PUBMED 2659436 REFERENCE 2 (bases 1 to 4970) AUTHORS Christianson,T.W., Sikorski,R.S., Dante,M., Shero,J.H. and Hieter,P. TITLE Multifunctional yeast high-copy-number shuttle vectors JOURNAL Gene 110 (1), 119-122 (1992) PUBMED 1544568 REFERENCE 3 (bases 1 to 4970) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (22-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank accession U03447. FEATURES Location/Qualifiers source 1..4970 /organism="Cloning vector pRS413" /mol_type="other DNA" gene 504..1163 /gene="HIS3" CDS 504..1163 /gene="HIS3" /codon_start=1 /product="imidazoleglycerol-phosphate dehydratase" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVA EQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQ AFKEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIP HFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVL M" rep_origin complement(1423..1878) /note="f1 origin of replication (+ -)" gene complement(1661..2239) /gene="lacZalpha" CDS complement(1661..2239) /gene="lacZalpha" /codon_start=1 /product="beta-galactosidase alpha fragment" /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD" misc_feature 2076..2183 /gene="lacZalpha" /note="multiple cloning site (KpnI-SacI)" rep_origin complement(2637..3224) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point); rop gene has been disrupted" gene complement(3396..4256) /gene="bla" CDS complement(3396..4256) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(4188..4256) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein" misc_structure 4397..4515 /note="CEN6 (yeast chromosome VI centromere sequence)" rep_origin 4528..4901 /note="ARS209; histone H4 autonomously replicating sequence (ARS)" BASE COUNT 1331 a 1140 c 1170 g 1329 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg 2101 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag 2161 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc 2221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 2281 cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa 2341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 2401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 2461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 2521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 2581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 2641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 2701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 2761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 2821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 2881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 2941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 3001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 3061 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 3121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 3181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 3241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 3301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 3361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 3421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 3481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 3541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 3601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 3661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 3721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 3781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 3841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 3901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 3961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 4021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 4081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 4141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 4201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 4261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 4321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 4381 ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat 4441 aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt 4501 ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc 4561 ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca 4621 cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct 4681 gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc 4741 tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa 4801 atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca 4861 ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag aaaccattat 4921 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc //