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Uniformity of Coverage across ERCC-00004 and ERCC-00074
Dir_RNA_Transcript_Figure7
Coverage across transcripts ERCC-00004 (A) and ERCC-00074 (B) was assessed by mapping reads directly to the ERCC sequences and assessing coverage using bedtools cov in 100 bins along the transcript length. Libraries prepared using the NEBNext Ultra II Directional RNA Kit provided superior coverage across the transcripts.

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Available Kits:

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NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.
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NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads
Includes optimized mixes for directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.