NEB® Turbo Competent E. coli (High Efficiency) | NEB

Home Competent Cells Products NEB^® Turbo Competent E. coli (High Efficiency)

Catalog #	Concentration	Size	List Price	Your Price	Quantity
C2984H	Not Applicable	20 x 0.05 ml	$301.00	Sign In Sign In
C2984I	Not Applicable	6 x 0.2 ml	$228.00	Sign In Sign In

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Product Information

Chemically competent E. coli cells suitable for high efficiency transformation and rapid colony growth.

Highlights

Tight control of expression by lacl^q allows potentially toxic genes to be cloned
Highest growth rate on agar plates - visible colonies 6.5 hours after transformation
Activity nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
Resistance to phage T1 (fhuA2)
Suitable for blue/white screening by α-complementation of the β-galactosidase gene
Suitable for 5 minute transformation protocol with Amp^Rplasmids
EcoKr^-m^-, McrBC^-
K12 Strain
Isolate DNA after 4 hours of growth from a single overnight colony
Free of animal products

Transformation Efficiency

1 - 3 x 10⁹ cfu/μg pUC19 DNA

Genotype

F' proA⁺B⁺ lacI^q ∆lacZM15 / fhuA2 ∆(lac-proAB) glnV galK16 galE15 R(zgb-210::Tn10)Tet^S endA1 thi-1 ∆(hsdS-mcrB)5

This product is related to the following categories:: Cloning Competent Cell Strains Products

This product can be used in the following applications:: Transformation,; High-throughput cloning and automation solutions,; Fast Cloning: Accelerate your cloning workflows with reagents from NEB

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection	Working Concentration
Ampicillin	100 µg/ml
Carbenicillin	100 µg/ml
Chloramphenicol	33 µg/ml
Kanamycin	30 µg/ml
Streptomycin	25 µg/ml
Tetracycline	15 µg/ml

Shipping Notes

Ships on dry ice

Antibiotic Resistance

nit

Protocols, Manuals & Usage
- Protocols
  High Efficiency Transformation Protocol (C2984)
  
  5 Minute Transformation Protocol (C2984)
- Usage Guidelines
  Additional E. coli Strain Genotypes
  
  Chemical Transformation Tips
  
  Competent E. coli Genome Sequences Tool
  
  Genetic Markers
  
  McrA, McrBC and EcoKI Strain Phenotypes
  
  Restriction of Foreign DNA by E. coli K-12
- Application Notes
  Enhancing Transformation Efficiency
Tools & Resources
- Selection Charts
  Characteristics of Select E.coli Strains
  
  Competent Cell Product Comparison
  
  Competent Cell Selection Guide
- Web Tools
  Competitor Cross-Reference Tool
  
  NEBcloner^®
FAQs & Troubleshooting
- FAQs
  Which competent cell strains are compatible with Gateway^® Cloning?
  
  Does plasmid size affect colony size (C2984)?
  
  How long can I purify plasmid after the inoculation from a single colony using NEB Turbo competent E.coli (C2984)?
  
  How long should I incubate cells on ice after DNA has been added (NEB #C2984H and NEB #C2984I)?
  
  What are the strain properties (C2984)?
  
  What does the growth curve of NEB Turbo cells look like compared to DH5α?
  
  What is the difference between NEB #C2984H and NEB #C2984I?
  
  What is the optimal heat shock time for this strain (NEB #C2984H and NEB #C2984I)?
  
  Which strain of Competent E.coli should I use for general cloning?
  
  How should I calculate the transformation efficiency (C2984)?
  
  What are the solutions/recipes (C2984)?
  
  Can I store competent cells at -20°C instead of -80°C?
  
  Which kind of transformation tubes should be used?
  
  What volume of DNA can be added into competent cells?
  
  What is the shelf life for this strain (NEB #C2984H and NEB #C2984I)?
  
  Are NEB's competent cells compatible with the “Mix & Go" protocol?
  
  How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
  
  How should fragments be prepared for assembly using NEBuilder HiFi?
  
  Are NEB Turbo Competent E. coli (High Efficiency) or NEB Turbo Electrocompetent E. coli recommended for cloning repetitive or unstable DNA?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz

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