Home DNA Modifying Enzymes and Cloning Technologies Products Thermostable Inorganic Pyrophosphatase

Thermostable Inorganic Pyrophosphatase
recombinant No

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate and can be used for the enhancement of DNA replication. This enzyme retains 100% activity after incubation at 100°C for 4 hours.
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Catalog # Concentration Size List Price Your Price Quantity
M0296S 2,000 units/ml 250 units $82.00
M0296L 2,000 units/ml 1,250 units $333.00
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  • Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
    P2O7-4 + H2O -> 2HPO4-2.

    Product Source

    An E. coli strain carrying a plasmid encoding a pyrophosphatase from the extreme thermophile Thermococcus litoralis.
    This product is related to the following categories:
    Phosphatases Products,
    RNA Modification

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).

      Storage Buffer

      20 mM Tris-HCl
      100 mM KCl
      1 mM DTT
      0.1 mM EDTA
      50% Glycerol
      pH 8 @ 25°C

      Heat Inactivation

      No

    • Related Products

      Companion Products

    • Product Notes

      1. While a specific NEBuffer is not supplied, Thermostable Inorganic Pyrophosphatase works well in any buffer containing Mg++ (1-10 mM). This enzyme is typically used in PCR reactions, where the Mg++ concentration falls within this range.

    • References

      1. Heinonen, J.K. and Lahti, R.J. (1981). Analytical Biochemistry. 113, 313-317.

  • FAQs & Troubleshooting

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