Discovery of novel exonucleases and structure specific endonucleases

Exonucleases are a broad group of enzymes that degrade polynucleotides from ends. We are using high throughput enzymology methods, including capillary electrophoresis, to investigate the kinetics of these enzymes and profile their activity on modified oligonucleotide substrates. We are also screening metagenomic libraries for exonucleases with interesting properties and novel structure-specific endonucleases.