Amplification of GC-rich DNA targets can be difficult for most traditional polymerases. For standard PCR needs, OneTaq DNA polymerase was developed with both standard and GC buffers to provide high yield and specificity from AT-rich through to moderately GC-rich targets.
For particularly difficult amplicons, up to 80% GC, the OneTaq High GC enhancer should be added to the GC buffer. As with most GC enhancers, this component is not recommended on AT rich targets.
Q5 provides robust GC coverage with ultra low error rates and high processivity to set a new benchmark in high-fidelity amplification.
Both OneTaq and Q5 polymerases are also available as hot start enzymes. NEB's unique aptamer based hot start polymerases offer convenient room temperature reaction set up and unlike other hot start methods, they don't require a specific activation step.
At NEB, we have performed tens of thousands of PCR experiments, optimizing our polymerases, so you don't have to.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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